Feulgen microdensitometry is based on the affinity of this dye and the dna. In the first part of the study, smears were stained with hematoxylin eosin he, toluidin. Feulgen densitometric dna measurements were carried out on a leitz. Keywords c value, dna content, feulgen densitometry, genome size. Flow cytometric measurements of nuclear dna content were. In the cell nucleus is about 99% of the cellular dna, containing the genetic. A new extraction method for the quantitative determination of the fuchsin contained in a feulgen stained nucleoprotein sample has been introduced. Finally, nonhaemocyte nuclei in tissues were removed by manual image editing. Feulgen stain is a staining technique discovered by robert feulgen and used in histology to identify chromosomal material or dna in cell specimens. Analysis of nuclear dna content in capsicum solanaceae by. The study of genome size variation is important from a number of. Finally, in more severe conditions, a further ploidy peak of 7.
An analytic aproach policy for growth and expansion mcgraw hill. Details of how to carry out these general instructions should be available in the users manual. Standardization of cytogenetic techniques squash for counting of mitotic chromosomes in rubus. In feulgen dna densitometry, reference cells from haploid nuclei are. Staining affinities and cytochemical properties of the. The demonstration of theileria parva infection in intact. Comet bioassay is able to determine an exact way the value of sperm dna integrity. Tb is a basic dye that is used to identify dna, rna and gags at ph 4. Nuclear morphometry and ploidy of normal and neoplastic.
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